Hotspot

rpl16

LSC · 1548-2949 · 1402 bp

Back to Helichrysum

Pi 0.00573

MarkerSeek score 56.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1402
variable_sites: 8
pi: 0.00573
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GTCGAGGGAATTGCACGTAT	CGCACCAAAAACTCGAGTTC	50.0	50.0	58.1	58.0	2463-2465 bp	1.0	80.1
rpl16_p2	GTCGAGGGAATTGCACGTAT	ACACATCAATTCTCGAGCCC	50.0	50.0	58.1	58.0	2223-2225 bp	1.0	80.1
rpl16_p3	GTCGAGGGAATTGCACGTAT	TCGACGATTGCTAGCTCCTA	50.0	50.0	58.1	58.0	2253-2255 bp	1.0	80.0
rpl16_p4	GTCGAGGGAATTGCACGTAT	GCGTCTTTGATCACAGCAAC	50.0	50.0	58.1	58.0	2297-2299 bp	1.0	80.0
rpl16_p5	GCAAATTGCAGGACGTATCG	CGCACCAAAAACTCGAGTTC	50.0	50.0	57.9	58.0	2146-2148 bp	1.0	79.9