Hotspot

trnL-UAG-ccsA

SSC · 113494-113606 · 113 bp

Back to Helichrysum

Pi 0.0177

MarkerSeek score 61.40

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 113
variable_sites: 2
pi: 0.0177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TGAAATCGGTAGACACGCTG	TGATTTCATTGCCAAATAAGGT	50.0	31.8	58.0	54.6	286-295 bp	1.0	40.6
trnL-UAG-ccsA_p2	TAGACACGCTGCTCTTAGGA	TGATTTCATTGCCAAATAAGGT	50.0	31.8	57.9	54.6	277-286 bp	1.0	40.6
trnL-UAG-ccsA_p3	GGTAGACACGCTGCTCTTAG	TGATTTCATTGCCAAATAAGGT	55.0	31.8	57.8	54.6	279-288 bp	1.0	40.6
trnL-UAG-ccsA_p4	GTAGACACGCTGCTCTTAGG	TGATTTCATTGCCAAATAAGGT	55.0	31.8	57.8	54.6	278-287 bp	1.0	40.6
trnL-UAG-ccsA_p5	GTGAAATCGGTAGACACGCT	TGATTTCATTGCCAAATAAGGT	50.0	31.8	58.0	54.6	287-296 bp	1.0	40.5