Hotspot

rpl32-trnL-UAG

SSC · 112597-113413 · 817 bp

Back to Helichrysum

Pi 0.0162
MarkerSeek score 59.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
817
variable_sites
13
pi
0.0162
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCTCTTTCTACCGGGAATTCA AGCGTGTCTACCGATTTCAC 42.9 50.0 56.1 58.0 903 bp 1.0 59.1
rpl32-trnL-UAG_p2 TCTCTTTCTACCGGGAATTCA CAGCGTGTCTACCGATTTCA 42.9 50.0 56.1 58.0 904 bp 1.0 59.1
rpl32-trnL-UAG_p3 TCTCTTTCTACCGGGAATTCA TCCTAAGAGCAGCGTGTCTA 42.9 50.0 56.1 57.9 913 bp 1.0 58.2
rpl32-trnL-UAG_p4 TCTCTTTCTACCGGGAATTCA CCTAAGAGCAGCGTGTCTAC 42.9 55.0 56.1 57.8 912 bp 1.0 57.6
rpl32-trnL-UAG_p5 TCTCTTTCTACCGGGAATTCA CTAAGAGCAGCGTGTCTACC 42.9 55.0 56.1 57.8 911 bp 1.0 57.6