Hotspot

psbA-trnH-GUG

LSC · 83738-84145 · 408 bp

Back to Helichrysum

Pi 0.0354

MarkerSeek score 77.80

Primer available yes

Label psbA-trnH-GUG

Feature Metadata

label_name: psbA-trnH-GUG
feature_type: igs
region: LSC
length_bp: 408
variable_sites: 14
pi: 0.0354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnH-GUG_p1	CCGTGCTAACCTTGGTATGG	GATCCACTTGGCTACATCCG	55.0	55.0	58.3	58.1	517-527 bp	1.0	80.3
psbA-trnH-GUG_p2	CCGTGCTAACCTTGGTATGG	CAATCCACTGCCTTGATCCA	55.0	50.0	58.3	57.9	531-541 bp	1.0	80.1
psbA-trnH-GUG_p3	CCGTGCTAACCTTGGTATGG	AATCCACTGCCTTGATCCAC	55.0	50.0	58.3	57.9	530-540 bp	1.0	80.1
psbA-trnH-GUG_p4	CCGTGCTAACCTTGGTATGG	ACAATCCACTGCCTTGATCC	55.0	50.0	58.3	57.9	532-542 bp	1.0	80.1
psbA-trnH-GUG_p5	ACCGTGCTAACCTTGGTATG	GATCCACTTGGCTACATCCG	50.0	55.0	57.6	58.1	518-528 bp	1.0	79.8