Hotspot

psbD-trnG-UCC

LSC · 51805-53468 · 1664 bp

Back to Helichrysum

Pi 0.0074

MarkerSeek score 65.10

Primer available yes

Label psbD-trnG-UCC

Feature Metadata

label_name: psbD-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1664
variable_sites: 12
pi: 0.0074
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnG-UCC_p1	GAAACGGTCCCTACGTAACC	AATCAAACCGAAAGACCCCT	55.0	45.0	58.0	57.0	1825-1866 bp	1.0	74.6
psbD-trnG-UCC_p2	GAAACGGTCCCTACGTAACC	ATCAAACCGAAAGACCCCTT	55.0	45.0	58.0	57.0	1824-1865 bp	1.0	74.6
psbD-trnG-UCC_p3	GAAACGGTCCCTACGTAACC	GAATCAAACCGAAAGACCCCT	55.0	47.6	58.0	58.2	1826-1867 bp	1.0	72.7
psbD-trnG-UCC_p4	ACAAAAACGAAACGGTCCCT	AATCAAACCGAAAGACCCCT	45.0	45.0	58.2	57.0	1833-1874 bp	1.0	72.6
psbD-trnG-UCC_p5	ACAAAAACGAAACGGTCCCT	ATCAAACCGAAAGACCCCTT	45.0	45.0	58.2	57.0	1832-1873 bp	1.0	72.6