Hotspot

ndhJ-trnF-GAA

LSC · 35371-35971 · 601 bp

Back to Helichrysum

Pi 0.0116

MarkerSeek score 65.40

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 601
variable_sites: 7
pi: 0.0116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	TGGATAGGATGGCCTTTACG	AATAGCCGGGATAGCTCAGT	50.0	50.0	56.8	58.0	747-749 bp	1.0	70.8
ndhJ-trnF-GAA_p2	GGATAGGATGGCCTTTACGT	AATAGCCGGGATAGCTCAGT	50.0	50.0	56.8	58.0	746-748 bp	1.0	70.8
ndhJ-trnF-GAA_p3	TGGATAGGATGGCCTTTACG	CCTCGTGTCACCAGTTCAAA	50.0	50.0	56.8	58.1	704-706 bp	1.0	70.6
ndhJ-trnF-GAA_p4	GGATAGGATGGCCTTTACGT	CCTCGTGTCACCAGTTCAAA	50.0	50.0	56.8	58.1	703-705 bp	1.0	70.6
ndhJ-trnF-GAA_p5	TGAAAGTTGGATAGGATGGCC	AATAGCCGGGATAGCTCAGT	47.6	50.0	57.7	58.0	754-756 bp	1.0	70.2