Hotspot

rbcL

LSC · 54678-56135 · 1458 bp

Back to Helianthus

Pi 0.00381

MarkerSeek score 44.40

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1458
variable_sites: 21
pi: 0.00381
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	ACGCCTAATTCATGTCGAGT	TGGGCCGAGTTTCATTGAAA	45.0	45.0	57.0	58.0	1569-1578 bp	1.0	73.3
rbcL_p2	ACGCCTAATTCATGTCGAGT	TTGGGCCGAGTTTCATTGAA	45.0	45.0	57.0	58.0	1570-1579 bp	1.0	73.3
rbcL_p3	ACGCCTAATTCATGTCGAGT	ATTGGGCCGAGTTTCATTGA	45.0	45.0	57.0	57.8	1571-1580 bp	1.0	71.7
rbcL_p4	ACGCCTAATTCATGTCGAGT	AAAGATTGGGCCGAGTTTCA	45.0	45.0	57.0	57.7	1575-1584 bp	1.0	71.1
rbcL_p5	AACGCCTAATTCATGTCGAGT	TGGGCCGAGTTTCATTGAAA	42.9	45.0	57.8	58.0	1570-1579 bp	1.0	71.0