Hotspot

psaC

SSC · 119132-119377 · 246 bp

Back to Helianthus

Pi 0.000439

MarkerSeek score 23.50

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 2
pi: 0.000439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	AAAACTCGGGGAACTCATCG	TGGACTCAGTTTCTGTAATGT	50.0	38.1	57.9	54.5	374-376 bp	0.972973	43.9
psaC_p2	CTCGGGGAACTCATCGATTC	TGGACTCAGTTTCTGTAATGT	55.0	38.1	57.9	54.5	370-372 bp	0.972973	43.5
psaC_p3	AAAACTCGGGGAACTCATCG	ATGGACTCAGTTTCTGTAATGT	50.0	36.4	57.9	55.1	375-377 bp	0.972973	40.6
psaC_p4	AACTCGGGGAACTCATCGAT	TGGACTCAGTTTCTGTAATGT	50.0	38.1	58.5	54.5	372-374 bp	0.972973	40.6
psaC_p5	ACTCGGGGAACTCATCGATT	TGGACTCAGTTTCTGTAATGT	50.0	38.1	58.5	54.5	371-373 bp	0.972973	40.6