Hotspot

petB

LSC · 74352-75768 · 1417 bp

Back to Helianthus

Pi 0.00207

MarkerSeek score 41.10

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1417
variable_sites: 18
pi: 0.00207
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CCCCCAAGTCCTAGCTTTTC	AGTTCCTACCCCAAGTGATA	55.0	45.0	57.9	54.8	1591-1597 bp	1.0	54.5
petB_p2	CCCCCAAGTCCTAGCTTTTC	ATAGTTCCTACCCCAAGTGA	55.0	45.0	57.9	54.8	1593-1599 bp	1.0	54.5
petB_p3	CCCCCAAGTCCTAGCTTTTC	TAGTTCCTACCCCAAGTGAT	55.0	45.0	57.9	54.8	1592-1598 bp	1.0	54.5
petB_p4	TTTTGGTAGTTCGACCGTGG	AGTTCCTACCCCAAGTGATA	50.0	45.0	58.1	54.8	1526-1532 bp	1.0	54.4
petB_p5	TTTTGGTAGTTCGACCGTGG	TAGTTCCTACCCCAAGTGAT	50.0	45.0	58.1	54.8	1527-1533 bp	1.0	54.4