Hotspot

ndhD

SSC · 119484-120986 · 1503 bp

Back to Helianthus

Pi 0.000775

MarkerSeek score 33.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 13
pi: 0.000775
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCACAGGTTTTTCTGGTCCA	TTCATTAGCCAACCAACTCT	50.0	40.0	58.6	54.5	1590-1596 bp	1.0	48.5
ndhD_p2	GCACAGGTTTTTCTGGTCCA	TTTCATTAGCCAACCAACTCT	50.0	38.1	58.6	55.4	1591-1597 bp	1.0	47.4
ndhD_p3	TTGGGCACAGGTTTTTCTGG	TTCATTAGCCAACCAACTCT	50.0	40.0	58.9	54.5	1594-1600 bp	1.0	46.3
ndhD_p4	GCACAGGTTTTTCTGGTCCA	TTTTCATTAGCCAACCAACTCT	50.0	36.4	58.6	56.2	1592-1598 bp	1.0	45.5
ndhD_p5	TTTGGGCACAGGTTTTTCTG	TTCATTAGCCAACCAACTCT	45.0	40.0	57.0	54.5	1595-1601 bp	1.0	45.5