Hotspot

trnR-ACG-rrn5

IRa · 128015-128290 · 276 bp

Back to Helianthus

Pi 0.00826
MarkerSeek score 55.40
Primer available yes
Label trnR-ACG-rrn5

Feature Metadata

label_name
trnR-ACG-rrn5
feature_type
igs
region
IRa
length_bp
276
variable_sites
7
pi
0.00826
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn5_p1 CCACGTGCTCTAATCCTCTG GGTGACGATACTGTAGGGGA 55.0 55.0 57.8 57.9 336-430 bp 1.0 83.3
trnR-ACG-rrn5_p2 CCACGTGCTCTAATCCTCTG AATCCATCCCGAACTTGGTG 55.0 50.0 57.8 57.9 371-465 bp 1.0 82.7
trnR-ACG-rrn5_p3 CCACGTGCTCTAATCCTCTG ACCAATCCATCCCGAACTTG 55.0 50.0 57.8 57.9 374-468 bp 1.0 82.7
trnR-ACG-rrn5_p4 CCACGTGCTCTAATCCTCTG CAATCCATCCCGAACTTGGT 55.0 50.0 57.8 57.9 372-466 bp 1.0 82.7
trnR-ACG-rrn5_p5 CCACGTGCTCTAATCCTCTG CACCAATCCATCCCGAACTT 55.0 50.0 57.8 57.9 375-469 bp 1.0 82.6