Hotspot

rpl32-ndhF

SSC · 123337-124185 · 849 bp

Back to Helianthus

Pi 0.00227

MarkerSeek score 49.80

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 849
variable_sites: 19
pi: 0.00227
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CGCCCCATATCCTTTCCTTT	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.6	58.0	1026-1038 bp	1.0	79.7
rpl32-ndhF_p2	CGCCCCATATCCTTTCCTTT	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	57.6	58.0	1024-1036 bp	1.0	79.6
rpl32-ndhF_p3	CGCCCCATATCCTTTCCTTT	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	57.6	58.0	1025-1037 bp	1.0	79.6
rpl32-ndhF_p4	TGACAAAGCTTTTAACGCCG	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.6	58.0	1044-1056 bp	1.0	79.4
rpl32-ndhF_p5	TGACAAAGCTTTTAACGCCG	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.6	58.0	1042-1054 bp	1.0	79.3