Hotspot

trnL-UAG-rpl32

SSC · 122409-123171 · 763 bp

Back to Helianthus

Pi 0.00377
MarkerSeek score 56.90
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
763
variable_sites
21
pi
0.00377
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC AAAGGAAAGGATATGGGGCG 50.0 50.0 58.0 57.6 891-924 bp 1.0 80.4
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA AAAGGAAAGGATATGGGGCG 50.0 50.0 58.0 57.6 892-925 bp 1.0 80.4
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 873-906 bp 1.0 80.2
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 874-907 bp 1.0 80.2
trnL-UAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA AAAGGAAAGGATATGGGGCG 50.0 50.0 57.9 57.6 901-934 bp 1.0 79.4