Hotspot

ndhD-ccsA

SSC · 120987-121251 · 265 bp

Back to Helianthus

Pi 0.0204
MarkerSeek score 59.70
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
265
variable_sites
20
pi
0.0204
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 AGGTATTGGTATGTACCCCGA TGTGGCAACTCTAGGCTTTC 47.6 50.0 57.7 57.8 450-475 bp 1.0 72.6
ndhD-ccsA_p2 TCAGTTGACAAGGTTGAAGGT TGTGGCAACTCTAGGCTTTC 42.9 50.0 57.4 57.8 413-438 bp 1.0 70.5
ndhD-ccsA_p3 AGGTATTGGTATGTACCCCGA GTGGCAACTCTAGGCTTTCT 47.6 50.0 57.7 57.5 449-474 bp 1.0 70.2
ndhD-ccsA_p4 AGGTATTGGTATGTACCCCGA AATTCCGCAATTGTGGCAAC 47.6 45.0 57.7 58.2 467 bp 0.027027 28.8
ndhD-ccsA_p5 AGGTATTGGTATGTACCCCGA CAAATTCCGCAATTGTGGCA 47.6 45.0 57.7 58.2 469 bp 0.027027 28.8