Hotspot

petA-psbJ

LSC · 62627-63429 · 803 bp

Back to Helianthus

Pi 0.00351

MarkerSeek score 58.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 20
pi: 0.00351
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGTTCTTCTTCGCAGCTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.0	57.6	900-928 bp	1.0	81.4
petA-psbJ_p2	TTGTTCTTCTTCGCAGCTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.0	57.6	901-929 bp	1.0	81.4
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.9	57.6	907-935 bp	1.0	81.4
petA-psbJ_p4	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.0	57.6	917-945 bp	1.0	81.3
petA-psbJ_p5	TGTTCTTCTTCGCAGCTGTT	TGTGATCGGTTTAGTAGGCG	45.0	50.0	58.0	57.4	938-966 bp	1.0	79.6