Hotspot

accD-psaI

LSC · 58145-58842 · 698 bp

Back to Helianthus

Pi 0.00182

MarkerSeek score 54.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 15
pi: 0.00182
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAAGCGTTCTGAGCGAGT	AAAGAAGCCATTGCGATTGC	45.0	45.0	58.4	58.0	834-855 bp	1.0	82.1
accD-psaI_p2	AAAAGCGTTCTGAGCGAGTT	AAAGAAGCCATTGCGATTGC	45.0	45.0	58.4	58.0	833-854 bp	1.0	82.1
accD-psaI_p3	AAAGCGTTCTGAGCGAGTTA	AAAGAAGCCATTGCGATTGC	45.0	45.0	57.6	58.0	832-853 bp	1.0	81.9
accD-psaI_p4	AAGCGTTCTGAGCGAGTTAT	AAAGAAGCCATTGCGATTGC	45.0	45.0	57.3	58.0	831-852 bp	1.0	80.2
accD-psaI_p5	AGCGTTCTGAGCGAGTTATT	AAAGAAGCCATTGCGATTGC	45.0	45.0	57.3	58.0	830-851 bp	1.0	80.2