Hotspot

rps16

LSC · 5105-6227 · 1123 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1123
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 CCCACCCTCTTCTCTCTCTC TCTCTGAAACGATCGAACAA 60.0 40.0 58.2 54.8 1249 bp 1.0 52.5
rps16_p2 CCCACCCTCTTCTCTCTCTC TTCTCTGAAACGATCGAACA 60.0 40.0 58.2 54.8 1250 bp 1.0 52.5
rps16_p3 ATATGAATCACCCCCACCCT TCTCTGAAACGATCGAACAA 50.0 40.0 57.8 54.8 1261 bp 1.0 52.4
rps16_p4 ATATGAATCACCCCCACCCT TTCTCTGAAACGATCGAACA 50.0 40.0 57.8 54.8 1262 bp 1.0 52.4
rps16_p5 CCCACCCTCTTCTCTCTCTC TCTGAAACGATCGAACAAATGAC 60.0 39.1 58.2 57.7 1247 bp 1.0 52.1