Hotspot

matK

LSC · 2001-3503 · 1503 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1503
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 CAACCCTTACTCTTCCTGCA ACCCTGTTTTGACTATATCGCA 50.0 40.9 57.1 57.5 1706 bp 1.0 53.3
matK_p2 ACCCTTACTCTTCCTGCATG ACCCTGTTTTGACTATATCGCA 50.0 40.9 56.9 57.5 1704 bp 1.0 51.4
matK_p3 TCAACCCTTACTCTTCCTGC ACCCTGTTTTGACTATATCGCA 50.0 40.9 56.8 57.5 1707 bp 1.0 50.9
matK_p4 CAACCCTTACTCTTCCTGCA CCCTGTTTTGACTATATCGCAC 50.0 45.5 57.1 57.2 1705 bp 1.0 50.8
matK_p5 AACCCTTACTCTTCCTGCATG ACCCTGTTTTGACTATATCGCA 47.6 40.9 57.6 57.5 1705 bp 1.0 49.3