Hotspot

accD

LSC · 60828-62303 · 1476 bp

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Pi 0
MarkerSeek score 38.40
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1476
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 TGGAAATAGGGACAAGAAAACTCT CGGATTTTGCTTTGATTCCGA 37.5 42.9 57.8 57.5 1576 bp 1.0 40.0
accD_p2 TGGAAATAGGGACAAGAAAACTCT TCGGATTTTGCTTTGATTCCG 37.5 42.9 57.8 57.5 1577 bp 1.0 40.0
accD_p3 TGGAAATAGGGACAAGAAAACTC CGGATTTTGCTTTGATTCCGA 39.1 42.9 56.4 57.5 1576 bp 1.0 40.0
accD_p4 TGGAAATAGGGACAAGAAAACTC TCGGATTTTGCTTTGATTCCG 39.1 42.9 56.4 57.5 1577 bp 1.0 40.0
accD_p5 TGGAAATAGGGACAAGAAAACTCT CGGATTTTGCTTTGATTCCG 37.5 45.0 57.8 56.0 1576 bp 1.0 40.0