Hotspot

ndhK-ndhC

LSC · 53115-53168 · 54 bp

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Pi 0
MarkerSeek score 54.30
Primer available yes
Label ndhK-ndhC

Feature Metadata

label_name
ndhK-ndhC
feature_type
igs
region
LSC
length_bp
54
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhK-ndhC_p1 AGCGGCCATAAACTTGAGAG ATGGCGAAAGGGAGCATTAG 50.0 50.0 58.0 58.0 194-195 bp 1.0 80.0
ndhK-ndhC_p2 AGCGGCCATAAACTTGAGAG GGCGAAAGGGAGCATTAGAA 50.0 50.0 58.0 58.0 192-193 bp 1.0 79.9
ndhK-ndhC_p3 AGCGGCCATAAACTTGAGAG TTTATGCATGGCGAAAGGGA 50.0 45.0 58.0 57.9 201-202 bp 1.0 79.1
ndhK-ndhC_p4 AGCGGCCATAAACTTGAGAG GTTTATGCATGGCGAAAGGG 50.0 50.0 58.0 57.8 202-203 bp 1.0 78.5
ndhK-ndhC_p5 TTTGGGTTGTTCGAGCAAGT ATGGCGAAAGGGAGCATTAG 45.0 50.0 58.2 58.0 127-128 bp 1.0 78.5