Hotspot

rpoB-trnC-GCA

LSC · 27866-29011 · 1146 bp

Back to Heimia

Pi 0.000873
MarkerSeek score 59.30
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1146
variable_sites
1
pi
0.000873
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCTTCATTTCCATCCCCGAG CCGGATTTGAACTGGGGAAA 55.0 50.0 58.0 58.1 1231 bp 1.0 79.2
rpoB-trnC-GCA_p2 CCTTCATTTCCATCCCCGAG TTGTTAATCAGGCGACACCC 55.0 50.0 58.0 58.2 1249 bp 1.0 78.4
rpoB-trnC-GCA_p3 CCTTCATTTCCATCCCCGAG AAAAGGATTTGCAGTCCCCC 55.0 50.0 58.0 58.4 1213 bp 1.0 77.0
rpoB-trnC-GCA_p4 TCCTTCATTTCCATCCCCGA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.4 58.1 1232 bp 1.0 76.1
rpoB-trnC-GCA_p5 TTCCTTCATTTCCATCCCCG CCGGATTTGAACTGGGGAAA 50.0 50.0 57.6 58.1 1233 bp 1.0 75.9