Hotspot

trnS-GCU-FNM11_pgt005

LSC · 8713-9434 · 722 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnS-GCU-FNM11_pgt005

Feature Metadata

label_name
trnS-GCU-FNM11_pgt005
feature_type
igs
region
LSC
length_bp
722
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-FNM11_pgt005_p1 CAATCCGACGCTTTAGTCCA TAGGAATCAAACCGAGGCAC 50.0 50.0 58.0 57.6 838 bp 1.0 76.8
trnS-GCU-FNM11_pgt005_p2 AATCCGACGCTTTAGTCCAC TAGGAATCAAACCGAGGCAC 50.0 50.0 58.0 57.6 837 bp 1.0 76.8
trnS-GCU-FNM11_pgt005_p3 GCTCGTACAACGGATTAGCA TAGGAATCAAACCGAGGCAC 50.0 50.0 58.1 57.6 856 bp 1.0 76.2
trnS-GCU-FNM11_pgt005_p4 ACGGAAAGAGAGGGATTCGA TAGGAATCAAACCGAGGCAC 50.0 50.0 58.2 57.6 892 bp 1.0 75.6
trnS-GCU-FNM11_pgt005_p5 AGCTCGTACAACGGATTAGC TAGGAATCAAACCGAGGCAC 50.0 50.0 57.8 57.6 857 bp 1.0 75.2