Hotspot

trnK-rps16

LSC · 4279-5104 · 826 bp

Back to Heimia

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-rps16

Feature Metadata

label_name
trnK-rps16
feature_type
igs
region
LSC
length_bp
826
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-rps16_p1 AAAGCCGAGTACTCTACCGT GCGCTCAACCTACAGAAACT 50.0 50.0 58.2 58.2 949 bp 1.0 77.0
trnK-rps16_p2 AAGCCGAGTACTCTACCGTT GCGCTCAACCTACAGAAACT 50.0 50.0 58.2 58.2 948 bp 1.0 77.0
trnK-rps16_p3 AAAGCCGAGTACTCTACCGT AAAAGGCGGGGGTTTTTACA 50.0 45.0 58.2 58.2 913 bp 1.0 77.0
trnK-rps16_p4 AAGCCGAGTACTCTACCGTT AAAAGGCGGGGGTTTTTACA 50.0 45.0 58.2 58.2 912 bp 1.0 77.0
trnK-rps16_p5 AAGCCGAGTACTCTACCGTT TTCTTGAAAAGGGCGCTCAA 50.0 45.0 58.2 58.3 960 bp 1.0 76.1