Hotspot

rps12-trnV-GAC

Genome · 92404-94517 · 2114 bp

Back to Hedysarum

Pi 0.0302

MarkerSeek score 76.10

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: Genome
length_bp: 2114
variable_sites: 297
pi: 0.0302
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	GAGGTTAAGCGTACTCTGGC	GATGACTTCCACCACGTCAA	55.0	50.0	58.1	57.8	1477-2226 bp	1.0	90.1
rps12-trnV-GAC_p2	GAGGTTAAGCGTACTCTGGC	AACTGATGACTTCCACCACG	55.0	50.0	58.1	57.8	1481-2230 bp	1.0	90.0
rps12-trnV-GAC_p3	GAGGTTAAGCGTACTCTGGC	ATGACTTCCACCACGTCAAG	55.0	50.0	58.1	57.8	1476-2225 bp	1.0	90.0
rps12-trnV-GAC_p4	GGTTAAGCGTACTCTGGCAA	GATGACTTCCACCACGTCAA	50.0	50.0	57.9	57.8	1475-2224 bp	1.0	90.0
rps12-trnV-GAC_p5	GGTTAAGCGTACTCTGGCAA	ATGACTTCCACCACGTCAAG	50.0	50.0	57.9	57.8	1474-2223 bp	1.0	89.9