Hotspot

trnG-UCC-trnS-GCU

Genome · 52011-52691 · 681 bp

Back to Hedysarum

Pi 0.0301

MarkerSeek score 75.10

Primer available yes

Label trnG-UCC-trnS-GCU

Feature Metadata

label_name: trnG-UCC-trnS-GCU
feature_type: igs
region: Genome
length_bp: 681
variable_sites: 104
pi: 0.0301
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnS-GCU_p1	GGATCCATAGAACGAATCGCA	GCTTTAGTCCACTCAGCCAT	47.6	50.0	58.0	57.6	489-822 bp	1.0	80.5
trnG-UCC-trnS-GCU_p2	GGATCCATAGAACGAATCGCA	AGTCCACTCAGCCATCTCTC	47.6	55.0	58.0	58.5	484-817 bp	1.0	79.7
trnG-UCC-trnS-GCU_p3	ACGAATCGCACTTTTACCACT	GCTTTAGTCCACTCAGCCAT	42.9	50.0	58.2	57.6	478-811 bp	1.0	78.8
trnG-UCC-trnS-GCU_p4	ACGAATCGCACTTTTACCAC	GCTTTAGTCCACTCAGCCAT	45.0	50.0	56.8	57.6	478-811 bp	1.0	78.8
trnG-UCC-trnS-GCU_p5	TCCATAGAACGAATCGCACT	GCTTTAGTCCACTCAGCCAT	45.0	50.0	56.8	57.6	486-819 bp	1.0	78.7