Hotspot

trnC-GCA-rpoB

Genome · 33018-34216 · 1199 bp

Back to Hedysarum

Pi 0.0221

MarkerSeek score 76.10

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: Genome
length_bp: 1199
variable_sites: 142
pi: 0.0221
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CCGGATTTGAACTGGGGAAA	TTCATTTCCAACCCCAAGCA	50.0	45.0	58.1	57.9	1189-1357 bp	1.0	90.6
trnC-GCA-rpoB_p2	CCGGATTTGAACTGGGGAAA	TCATTTCCAACCCCAAGCAT	50.0	45.0	58.1	57.7	1188-1356 bp	1.0	88.8
trnC-GCA-rpoB_p3	AAAAGGATTTGCAGTCCCCC	TTCATTTCCAACCCCAAGCA	50.0	45.0	58.4	57.9	1171-1339 bp	1.0	88.4
trnC-GCA-rpoB_p4	AAAAGGATTTGCAGTCCCCC	TCATTTCCAACCCCAAGCAT	50.0	45.0	58.4	57.7	1170-1338 bp	1.0	86.6
trnC-GCA-rpoB_p5	CCGGATTTGAACTGGGGAAA	CTTCATTTCCAACCCCAAGC	50.0	50.0	58.1	57.3	1190-1358 bp	1.0	85.3