Hotspot

petN-trnC-GCA

Genome · 32010-32946 · 937 bp

Back to Hedysarum

Pi 0.0257

MarkerSeek score 77.00

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: Genome
length_bp: 937
variable_sites: 139
pi: 0.0257
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.0	58.1	845-1063 bp	1.0	91.3
petN-trnC-GCA_p2	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	45.0	50.0	58.0	58.4	863-1081 bp	1.0	89.1
petN-trnC-GCA_p3	AGAGTCCACTTCTTCCCCAT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.6	58.1	899-1117 bp	1.0	88.5
petN-trnC-GCA_p4	CAGCCCAAGCAAGACTTACT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.8	58.1	908-1057 bp	0.92	86.6
petN-trnC-GCA_p5	CAGCCCAAGCAAGACTTACT	GGGGGACTGCAAATCCTTTT	50.0	50.0	57.8	58.4	926-1075 bp	0.92	84.4