Hotspot

trnS-UGA-psbC

Genome · 25999-26203 · 205 bp

Back to Hedysarum

Pi 0.0466

MarkerSeek score 76.80

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: Genome
length_bp: 205
variable_sites: 39
pi: 0.0466
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GGTTCGAATCCCCCTCTCT	ATTTATGGCACGCGGGAAG	57.9	52.6	58.1	58.6	306-341 bp	1.0	70.6
trnS-UGA-psbC_p2	TTCGAATCCCCCTCTCTCC	ATTTATGGCACGCGGGAAG	57.9	52.6	57.8	58.6	304-339 bp	1.0	69.4
trnS-UGA-psbC_p3	GGTTCGAATCCCCCTCTCTC	ATTTATGGCACGCGGGAAG	60.0	52.6	59.2	58.6	306-341 bp	1.0	69.3
trnS-UGA-psbC_p4	GTTCGAATCCCCCTCTCTCC	ATTTATGGCACGCGGGAAG	60.0	52.6	59.2	58.6	305-340 bp	1.0	69.3
trnS-UGA-psbC_p5	TTCGAATCCCCCTCTCTCCT	ATTTATGGCACGCGGGAAG	55.0	52.6	59.4	58.6	304-339 bp	1.0	68.3