Hotspot

trnG-GCC-psbZ

Genome · 24480-25381 · 902 bp

Back to Hedysarum

Pi 0.0715

MarkerSeek score 83.20

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: Genome
length_bp: 902
variable_sites: 310
pi: 0.0715
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	CGGATAGCGGGAATCGAAC	CTTCTCCTGATGGTTGGTCG	57.9	55.0	58.2	58.0	531-1087 bp	1.0	82.4
trnG-GCC-psbZ_p2	GATAGCGGGAATCGAACCC	CTTCTCCTGATGGTTGGTCG	57.9	55.0	57.8	58.0	529-1085 bp	1.0	82.2
trnG-GCC-psbZ_p3	GGATAGCGGGAATCGAACC	CTTCTCCTGATGGTTGGTCG	57.9	55.0	57.8	58.0	530-1086 bp	1.0	82.2
trnG-GCC-psbZ_p4	CGGATAGCGGGAATCGAAC	TTGCTTCTCCTGATGGTTGG	57.9	50.0	58.2	57.8	534-1090 bp	1.0	80.8
trnG-GCC-psbZ_p5	CGGATAGCGGGAATCGAAC	TCCTGATGGTTGGTCGAGTA	57.9	50.0	58.2	57.8	527-1083 bp	1.0	80.7