Hotspot

psbA-trnK-UUU

Genome · 1370-1703 · 334 bp

Back to Hedysarum

Pi 0.0424

MarkerSeek score 76.20

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: Genome
length_bp: 334
variable_sites: 62
pi: 0.0424
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TCGCGTCTCTCTAAAATTGCA	TGCAATGAAAAATGCACGCA	42.9	40.0	58.0	58.1	434-534 bp	1.0	82.5
psbA-trnK-UUU_p2	TCGCGTCTCTCTAAAATTGCA	GCAATGAAAAATGCACGCAC	42.9	45.0	58.0	57.8	433-533 bp	1.0	81.9
psbA-trnK-UUU_p3	CGCGTCTCTCTAAAATTGCAG	TGCAATGAAAAATGCACGCA	47.6	40.0	57.7	58.1	433-533 bp	1.0	80.5
psbA-trnK-UUU_p4	CGCGTCTCTCTAAAATTGCAG	GCAATGAAAAATGCACGCAC	47.6	45.0	57.7	57.8	432-532 bp	1.0	79.9
psbA-trnK-UUU_p5	TCGCGTCTCTCTAAAATTGCA	CAATGAAAAATGCACGCACG	42.9	45.0	58.0	57.5	432-532 bp	1.0	79.8