Hotspot

petD

LSC · 77261-78477 · 1217 bp

Back to Hedyotis

Pi 0.00493

MarkerSeek score 49.40

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1217
variable_sites: 9
pi: 0.00493
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTGAAGGGAATTCTCTGAAGA	GTCACTTTGACGCGACTAGT	39.1	50.0	57.2	58.0	1313-1357 bp	1.0	49.5
petD_p2	AGTTGAAGGGAATTCTCTGAAGA	GACGCGACTAGTCCCTAGAT	39.1	55.0	57.2	58.1	1305-1349 bp	1.0	48.9
petD_p3	AGTTGAAGGGAATTCTCTGAAGA	TTTGACGCGACTAGTCCCTA	39.1	50.0	57.2	58.2	1308-1352 bp	1.0	48.5
petD_p4	AGTTGAAGGGAATTCTCTGAAGA	TCACTTTGACGCGACTAGTC	39.1	50.0	57.2	57.7	1312-1356 bp	1.0	47.3
petD_p5	AGTTGAAGGGAATTCTCTGAAGAA	GTCACTTTGACGCGACTAGT	37.5	50.0	57.8	58.0	1313-1357 bp	1.0	46.6