Hotspot

ycf1-ndhF

SSC · 110508-110602 · 95 bp

Back to Hedyotis

Pi 0.186

MarkerSeek score 75.70

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 95
variable_sites: 21
pi: 0.186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GGAAGAAGGGGTGGAAAGTG	TTGATCGGCGTGTGATTGAT	55.0	45.0	58.1	58.0	479-512 bp	1.0	90.0
ycf1-ndhF_p2	AACCTCACAAGCGGAAGAAG	TTGATCGGCGTGTGATTGAT	50.0	45.0	58.1	58.0	491-524 bp	1.0	89.6
ycf1-ndhF_p3	AGAAACCTCACAAGCGGAAG	TTGATCGGCGTGTGATTGAT	50.0	45.0	58.1	58.0	494-527 bp	1.0	89.6
ycf1-ndhF_p4	GAAACCTCACAAGCGGAAGA	TTGATCGGCGTGTGATTGAT	50.0	45.0	58.1	58.0	493-526 bp	1.0	89.5
ycf1-ndhF_p5	AAGAGGGATCCACCGAAGAA	TTGATCGGCGTGTGATTGAT	50.0	45.0	58.0	58.0	399-432 bp	0.666667	70.5