Hotspot

accD-psaI

LSC · 58994-59658 · 665 bp

Back to Hedyotis

Pi 0.014

MarkerSeek score 66.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 14
pi: 0.014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGACCTAATCGTCCCACGTA	TTGCAAGTGCCGGAAATACT	50.0	45.0	57.9	58.1	832-911 bp	1.0	81.9
accD-psaI_p2	TGACCTAATCGTCCCACGTA	AAAGAAGCCATTGCAAGTGC	50.0	45.0	57.9	57.8	842-921 bp	1.0	81.4
accD-psaI_p3	TTTGACCTAATCGTCCCACG	TTGCAAGTGCCGGAAATACT	50.0	45.0	57.6	58.1	834-913 bp	1.0	79.9
accD-psaI_p4	TTTGACCTAATCGTCCCACG	AAAGAAGCCATTGCAAGTGC	50.0	45.0	57.6	57.8	844-923 bp	1.0	79.4
accD-psaI_p5	TGACCTAATCGTCCCACGTA	GCCGGAAATACTAGGCCTAC	50.0	55.0	57.9	57.5	824-903 bp	1.0	79.1