Hotspot

trnS-GGA-rps4

LSC · 45473-45814 · 342 bp

Back to Hedyotis

Pi 0.0409

MarkerSeek score 66.50

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 342
variable_sites: 15
pi: 0.0409
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	AGTCGTAGAATATTATTCTCGTCA	50.0	33.3	58.1	55.1	384-413 bp	1.0	47.0
trnS-GGA-rps4_p2	GGCTTTTGTTTACCGAGGGT	AGTCGTAGAATATTATTCTCGTCA	50.0	33.3	58.4	55.1	383-412 bp	1.0	47.0
trnS-GGA-rps4_p3	AGGCTTTTGTTTACCGAGGG	AGTCGTAGAATATTATTCTCGTCAA	50.0	32.0	58.1	55.8	384-413 bp	1.0	47.0
trnS-GGA-rps4_p4	AGGCTTTTGTTTACCGAGGG	GTCGTAGAATATTATTCTCGTCA	50.0	34.8	58.1	53.7	383-412 bp	1.0	47.0
trnS-GGA-rps4_p5	ATAGGAGAGATGGCCGAGTG	AGTCGTAGAATATTATTCTCGTCA	55.0	33.3	58.4	55.1	434-463 bp	1.0	46.7