Hotspot

trnR-UCU-atpA

LSC · 10686-10807 · 122 bp

Back to Hedyotis

Pi 0.022

MarkerSeek score 68.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 4
pi: 0.022
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AATCAGACGGAAAAGCGTCC	CTGAGGAAGCGCAATCTCTT	50.0	50.0	58.6	58.0	277-279 bp	1.0	77.1
trnR-UCU-atpA_p2	AATCAGACGGAAAAGCGTCC	GGAGCAACTGGACCGTTTTA	50.0	50.0	58.6	58.1	240-242 bp	1.0	76.6
trnR-UCU-atpA_p3	AATCAGACGGAAAAGCGTCC	GGACCGTTTTATCCTCGAGG	50.0	55.0	58.6	57.8	231-233 bp	1.0	75.8
trnR-UCU-atpA_p4	CGGAAAAGCGTCCATTGTCT	CTGAGGAAGCGCAATCTCTT	50.0	50.0	58.8	58.0	270-272 bp	1.0	74.9
trnR-UCU-atpA_p5	AGACGGAAAAGCGTCCATTG	CTGAGGAAGCGCAATCTCTT	50.0	50.0	58.8	58.0	273-275 bp	1.0	74.8