Hotspot

rps16-trnQ-UUG

LSC · 6065-7561 · 1497 bp

Back to Hedyotis

Pi 0.0113

MarkerSeek score 59.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1497
variable_sites: 24
pi: 0.0113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1382-1570 bp	1.0	83.6
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	AAAAATCGAAATGGGGCGTG	45.0	45.0	58.1	57.6	1441-1629 bp	1.0	81.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1386-1574 bp	1.0	78.8
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1412-1600 bp	1.0	78.7
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1374-1562 bp	1.0	78.4