Hotspot

ndhF

SSC · 129572-131797 · 2226 bp

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Pi 0.0137

MarkerSeek score 49.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 81
pi: 0.0137
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCGAGGCGAGGATAGAGTAT	TCCCCCTAGTTTGTCGACTT	55.0	50.0	57.9	58.0	2907-2930 bp	0.5	62.7
ndhF_p2	CCGAGGCGAGGATAGAGTAT	ATGGATATGGGATCGCCTGA	55.0	50.0	57.9	58.0	2840-2863 bp	0.5	62.6
ndhF_p3	CCGAGGCGAGGATAGAGTAT	TTTCACACATGCCCAAGTGA	55.0	45.0	57.9	57.9	2954-2977 bp	0.5	62.3
ndhF_p4	AACCAATAACCAAGCCCGAG	TCCCCCTAGTTTGTCGACTT	50.0	50.0	58.2	58.0	2940-2945 bp	0.375	55.8
ndhF_p5	AACCAATAACCAAGCCCGAG	ATGGATATGGGATCGCCTGA	50.0	50.0	58.2	58.0	2873-2878 bp	0.375	55.7