Hotspot

trnL-UAG-rpl32

SSC · 127727-128445 · 719 bp

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Pi 0.0253
MarkerSeek score 66.20
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
719
variable_sites
47
pi
0.0253
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC CTCGGGCTTGGTTATTGGTT 50.0 50.0 58.0 58.2 1406-1605 bp 0.625 68.1
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA CTCGGGCTTGGTTATTGGTT 50.0 50.0 58.0 58.2 1407-1606 bp 0.625 68.1
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC ATACTCTATCCTCGCCTCGG 50.0 55.0 58.0 57.9 1421-1592 bp 0.375 56.7
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA ATACTCTATCCTCGCCTCGG 50.0 55.0 58.0 57.9 1422-1593 bp 0.375 56.7
trnL-UAG-rpl32_p5 TACATTGTGTCCTGTCCCCT ATACTCTATCCTCGCCTCGG 50.0 55.0 58.0 57.9 1500-1671 bp 0.375 56.4