Hotspot

ycf4-cemA

LSC · 63635-64553 · 919 bp

Back to Hedyosmum

Pi 0.0158

MarkerSeek score 62.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 919
variable_sites: 37
pi: 0.0158
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGCGTGTACCGATGGAATT	ATATGGGAATGGGAGGGGG	45.0	57.9	57.9	57.8	1009-1020 bp	1.0	73.7
ycf4-cemA_p2	ACAAAAAGCTGCGGAATTGG	ATATGGGAATGGGAGGGGG	45.0	57.9	57.8	57.8	1037-1048 bp	1.0	73.2
ycf4-cemA_p3	AAGCTGCGGAATTGGCTTAT	ATATGGGAATGGGAGGGGG	45.0	57.9	58.2	57.8	1032-1043 bp	1.0	72.5
ycf4-cemA_p4	AGCTGCGGAATTGGCTTATT	ATATGGGAATGGGAGGGGG	45.0	57.9	58.2	57.8	1031-1042 bp	1.0	72.5
ycf4-cemA_p5	CTTGCGTGTACCGATGGAAT	ATATGGGAATGGGAGGGGG	50.0	57.9	58.3	57.8	1010-1021 bp	1.0	71.7