Hotspot

psaI-ycf4

LSC · 62629-63079 · 451 bp

Back to Hedyosmum

Pi 0.0303

MarkerSeek score 65.90

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 451
variable_sites: 34
pi: 0.0303
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	GCTAATGAACAGACCTGCGA	45.0	50.0	58.0	58.0	588-750 bp	1.0	88.2
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	AGCTAATGAACAGACCTGCG	45.0	50.0	58.0	58.0	589-751 bp	1.0	88.2
psaI-ycf4_p3	ATTTCCGGCAATTGCAATGG	TACGTCTGCCTGCTTGATTC	45.0	50.0	58.0	58.0	1075-1237 bp	1.0	87.4
psaI-ycf4_p4	ATTTCCGGCAATTGCAATGG	CAGCGGTATTACCAACGGAA	45.0	50.0	58.0	58.0	1201-1208 bp	0.625	67.9
psaI-ycf4_p5	ATTTCCGGCAATTGCAATGG	AGCGGTATTACCAACGGAAC	45.0	50.0	58.0	58.0	1200-1207 bp	0.625	67.9