Hotspot

psbM-trnD-GUC

LSC · 31177-32311 · 1135 bp

Back to Hedyosmum

Pi 0.0771

MarkerSeek score 86.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1135
variable_sites: 173
pi: 0.0771
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGCGAGAATATTGACTTCCA	GTTCGAGCCCCGTCAGTC	40.0	66.7	54.5	60.1	1032-1200 bp	1.0	52.0
psbM-trnD-GUC_p2	GGAACTAGAATGAACAATGCAGT	GTTCGAGCCCCGTCAGTC	39.1	66.7	57.1	60.1	1064-1232 bp	1.0	52.0
psbM-trnD-GUC_p3	ATGCGAGAATATTGACTTCCA	GTTCGAGCCCCGTCAGTC	38.1	66.7	55.1	60.1	1033-1201 bp	1.0	52.0
psbM-trnD-GUC_p4	TGCGAGAATATTGACTTCCAT	GTTCGAGCCCCGTCAGTC	38.1	66.7	55.1	60.1	1032-1200 bp	1.0	52.0
psbM-trnD-GUC_p5	AGAATGAACAATGCAGTAGC	GTTCGAGCCCCGTCAGTC	40.0	66.7	54.0	60.1	1058-1226 bp	1.0	52.0