Hotspot

atpF-atpH

LSC · 13687-14138 · 452 bp

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Pi 0.029

MarkerSeek score 71.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 452
variable_sites: 35
pi: 0.029
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GATGGCCAATAACCCAAGGA	AATTTACGGACTGGTCGTGG	50.0	50.0	57.5	57.9	554-573 bp	1.0	81.5
atpF-atpH_p2	GATGGCCAATAACCCAAGGA	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.5	58.3	540-559 bp	1.0	80.2
atpF-atpH_p3	GCCAATAACCCAAGGAAACG	AATTTACGGACTGGTCGTGG	50.0	50.0	57.4	57.9	550-569 bp	1.0	80.0
atpF-atpH_p4	TGGCCAATAACCCAAGGAAA	AATTTACGGACTGGTCGTGG	45.0	50.0	57.3	57.9	552-571 bp	1.0	79.5
atpF-atpH_p5	TTAAACCCGAAACTTCCGGC	AATTTACGGACTGGTCGTGG	50.0	50.0	58.8	57.9	575-594 bp	1.0	78.8