Hotspot

trnI-CAU-ycf2

SSC · 90288-90713 · 426 bp

Back to Hedychium

Pi 0.0648

MarkerSeek score 59.70

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: SSC
length_bp: 426
variable_sites: 26
pi: 0.0648
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.30
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	CTCAATTCGACGATCCAGGA	50.0	50.0	58.0	57.1	202-499 bp	1.0	85.0
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	TCTCAATTCGACGATCCAGG	50.0	50.0	58.0	57.1	203-500 bp	1.0	85.0
trnI-CAU-ycf2_p3	AGTTGGGCGCTTTAACCATT	CTCAATTCGACGATCCAGGA	45.0	50.0	58.4	57.1	203-500 bp	1.0	82.1
trnI-CAU-ycf2_p4	AGTTGGGCGCTTTAACCATT	TCTCAATTCGACGATCCAGG	45.0	50.0	58.4	57.1	204-501 bp	1.0	82.1
trnI-CAU-ycf2_p5	TTGGGCGCTTTAACCATTCA	CTCAATTCGACGATCCAGGA	45.0	50.0	58.4	57.1	201-498 bp	1.0	82.0