Hotspot

rps18-rpl20

LSC · 71674-71876 · 203 bp

Back to Hedychium

Pi 0.00542

MarkerSeek score 55.30

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 203
variable_sites: 11
pi: 0.00542
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	TCCCCCGAACTCGAATTACT	AAACAGAATTCCCCGGAGTG	50.0	50.0	58.1	57.8	257-277 bp	1.0	83.9
rps18-rpl20_p2	CCCCCGAACTCGAATTACTC	AAACAGAATTCCCCGGAGTG	55.0	50.0	57.8	57.8	256-276 bp	1.0	83.3
rps18-rpl20_p3	TCCCCCGAACTCGAATTACT	AACGAAACAGAATTCCCCGG	50.0	50.0	58.1	58.5	261-281 bp	1.0	81.6
rps18-rpl20_p4	TCCCCCGAACTCGAATTACT	GAAACAGAATTCCCCGGAGT	50.0	50.0	58.1	57.5	258-278 bp	1.0	81.5
rps18-rpl20_p5	CCCCCGAACTCGAATTACTC	AACGAAACAGAATTCCCCGG	55.0	50.0	57.8	58.5	260-280 bp	1.0	81.0