Hotspot

psbC-trnS-UGA

LSC · 37867-38008 · 142 bp

Back to Hedychium

Pi 0.0183

MarkerSeek score 60.70

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 10
pi: 0.0183
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CTTTCCATGACCCCTCTTCG	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.0	58.0	258-267 bp	1.0	84.1
psbC-trnS-UGA_p2	CATTTGTGGCATGCAGGAAG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	336-345 bp	1.0	83.3
psbC-trnS-UGA_p3	CATTTGTGGCATGCAGGAAG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.0	58.1	321-330 bp	1.0	83.2
psbC-trnS-UGA_p4	CATTTGTGGCATGCAGGAAG	GCTGAGTGGTTGATAGCTCC	50.0	55.0	58.0	58.1	328-337 bp	1.0	83.1
psbC-trnS-UGA_p5	CATTTGTGGCATGCAGGAAG	GGCTGAGTGGTTGATAGCTC	50.0	55.0	58.0	58.1	329-338 bp	1.0	83.1