Hotspot

petN-psbM

LSC · 29989-31359 · 1371 bp

Back to Hedychium

Pi 0.00281

MarkerSeek score 55.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1371
variable_sites: 17
pi: 0.00281
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1379-1487 bp	1.0	72.7
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1378-1486 bp	1.0	72.7
petN-psbM_p3	ATGGGGAAGGAGTGGACTTT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1376-1484 bp	1.0	71.8
petN-psbM_p4	ATTGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.3	57.6	1438-1546 bp	1.0	71.5
petN-psbM_p5	TGGGGAAGGAGTGGACTTTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1375-1483 bp	1.0	70.3