Hotspot

trnS-GCU-trnG-GCC

LSC · 8786-9531 · 746 bp

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Pi 0.0079
MarkerSeek score 57.30
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
746
variable_sites
26
pi
0.0079
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.95
markerseek_score
57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA CGGAGATACCCCTTCCCTTA 50.0 55.0 58.0 57.6 823-950 bp 1.0 83.2
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC CGGAGATACCCCTTCCCTTA 50.0 55.0 58.0 57.6 822-949 bp 1.0 83.2
trnS-GCU-trnG-GCC_p3 AGTATCAAGCGGGTCGAGTA CGGAGATACCCCTTCCCTTA 50.0 55.0 58.0 57.6 907-1034 bp 1.0 82.8
trnS-GCU-trnG-GCC_p4 GAGCGGAGAAAGAGGGATTC CGGAGATACCCCTTCCCTTA 55.0 55.0 57.8 57.6 879-1006 bp 1.0 81.3
trnS-GCU-trnG-GCC_p5 GTCGAGTAATCAGAGCGAGC CGGAGATACCCCTTCCCTTA 55.0 55.0 58.3 57.6 895-1022 bp 1.0 81.1