Hotspot

rps16-trnQ-UUG

LSC · 6000-7364 · 1365 bp

Back to Hedychium

Pi 0.00198
MarkerSeek score 55.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1365
variable_sites
21
pi
0.00198
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1447-1471 bp 1.0 82.3
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1445-1469 bp 1.0 82.3
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1453-1477 bp 1.0 82.2
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1429-1453 bp 1.0 81.5
rps16-trnQ-UUG_p5 CAGCCGATCATGTCCTTCAA CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 1451-1475 bp 1.0 81.3