Hotspot

rpl22

LSC · 87300-87725 · 426 bp

Back to Hedlundia

Pi 0.00407

MarkerSeek score 30.70

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 426
variable_sites: 4
pi: 0.00407
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TTGTCCCATAATCCCCCACT	TCCCGAGCGTCTACCATTAT	50.0	50.0	58.0	58.0	799-807 bp	1.0	82.9
rpl22_p2	CCCTTTTGTACCTGCCTGTT	TCCCGAGCGTCTACCATTAT	50.0	50.0	58.0	58.0	1267-1275 bp	1.0	82.2
rpl22_p3	CTCGGATCCATTCAACACGT	TCCCGAGCGTCTACCATTAT	50.0	50.0	58.0	58.0	1331-1339 bp	1.0	82.0
rpl22_p4	CGCCCTGCAATTTGTACTTG	TCCCGAGCGTCTACCATTAT	50.0	50.0	58.0	58.0	1290-1298 bp	1.0	82.0
rpl22_p5	CCCTTTTGTACCTGCCTGTT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1558-1566 bp	1.0	81.4