Hotspot

rps15-ycf1

SSC · 128711-129115 · 405 bp

Back to Hedlundia

Pi 0.00905

MarkerSeek score 36.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 405
variable_sites: 9
pi: 0.00905
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGATCCTCTGTTTTCGTCT	TGTATCCGCGATTGAAAATTCA	45.0	36.4	54.8	56.8	494-518 bp	1.0	41.8
rps15-ycf1_p2	CAGATCCTCTGTTTTCGTCTT	TGTATCCGCGATTGAAAATTCA	42.9	36.4	55.6	56.8	494-518 bp	1.0	41.8
rps15-ycf1_p3	CAGATCCTCTGTTTTCGTCTTT	TGTATCCGCGATTGAAAATTCA	40.9	36.4	56.4	56.8	494-518 bp	1.0	41.8
rps15-ycf1_p4	CAGATCCTCTGTTTTCGTCT	TCCGCGATTGAAAATTCATT	45.0	35.0	54.8	54.3	490-514 bp	1.0	41.8
rps15-ycf1_p5	CAGATCCTCTGTTTTCGTCT	TGTATCCGCGATTGAAAATTCAT	45.0	34.8	54.8	57.3	494-518 bp	1.0	41.8